Improved sensitivity of a thyrotropin receptor antibody assay.
نویسندگان
چکیده
Assay, Esther Jensen, Per Hyltoft Petersen, Ole Blaabjerg, Pia Skov Hansen, and Laszlo Hegedüs (Departments of 1 Clinical Biochemistry and 3 Endocrinology and Metabolism, Odense University Hospital, Odense, Denmark; 2 NOKLUS, Norwegian Quality Improvement of Primary Care Laboratories, Division for General Practice, University of Bergen, Bergen, Norway; * address correspondence to this author at: Department of Clinical Biochemistry, Odense University Hospital, DK-5000 Odense C, Denmark; fax 45-65-41-19-11, e-mail [email protected])
منابع مشابه
The measurement technologies of thyrotropin receptor antibodies from the past to the present
Thyroid Stimulating Hormone Receptor (TSH-R) autoantibodies are the main cause of Graveschr('39') disease and its external thyroid manifestations such as ophtalmopathy and dermatopathy. These antibodies are functionally different and are commonly called TSH receptor antibodies (TRAbs). In fact, TRAbs are a set of autoantibodies including TSHR-stimulating antibodies (TSAbs), TSHR- blocking antib...
متن کاملBridge Technology with TSH Receptor Chimera for Sensitive Direct Detection of TSH Receptor Antibodies Causing Graves' Disease: Analytical and Clinical Evaluation.
Graves' disease is caused by stimulating autoantibodies against the thyrotropin receptor inducing uncontrolled overproduction of thyroid hormones. A Bridge Assay is presented for direct detection of these thyroid-stimulating immunoglobulins using thyrotropin receptor chimeras. A capture receptor, formed by replacing aa residues 261-370 of the human thyrotropin receptor with residues 261-329 fro...
متن کاملTen commercial kits compared for assay of thyrotropin in the normal and thyrotoxic range.
I developed a standard procedure for assessing sensitivity, intra-assay precision, parallelism of sample dilutions, and assay drift, using a single trial assay kit. I used this to compare the performance of 10 commercial kits with an in-house radioimmunoassay for human thyrotropin. No one kit stood out as clearly superior overall. Differences in calibration between kits were evident in a compar...
متن کاملAnalytical and diagnostic accuracy of "second generation" assays for thyrotrophin receptor antibodies with radioactive and chemiluminescent tracers.
AIMS To investigate the analytical and diagnostic accuracy of thyrotrophin (TSH) receptor antibody assays using recombinant human TSH receptors. METHODS Sera from 68 patients with Graves' disease, 23 patients with autoimmune thyroiditis, and 119 healthy controls were evaluated in four different laboratories using both radioactive and chemiluminescent tracers. Functional sensitivity, interlabo...
متن کاملDiagnostic sensitivity of two radio receptor assays (TRAK Assay and TRAK Dyno Human) for detection of TSH receptor antibodies.
Radio receptor assays for the detection of TSH receptor antibodies in serum are typically based on binding the competition of TSH-R antibodies and (125)I "labeled" TSH for membrane preparation of thyrocytes (TBII tests). The sensitivity of the available tests utilizing porcine cell membranes was found to be around 80%. A new test (TRAK Dyno human, BRAHMS) utilizes human recombinant TSH receptor...
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عنوان ژورنال:
- Clinical chemistry
دوره 51 11 شماره
صفحات -
تاریخ انتشار 2005